%0 Book Section
%A Dabney, Jesse
%A Meyer, Matthias
%+ Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
%T Extraction of highly degraded DNA from ancient bones and teeth : 
%G eng
%U https://hdl.handle.net/21.11116/0000-0003-4B24-1
%R 10.1007/978-1-4939-9176-1_4
%D 2019
%* Review method: peer-reviewed
%X We provide a DNA extraction protocol optimized for the recovery of highly fragmented molecules preserved within bones and teeth. In this method, the hard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and the DNA is purified using spin columns with silica membranes. This method efficiently recovers molecules as short as 35 base-pairs long.
%K Ancient DNA ; DNA extraction ; Degraded DNA ; Silica purification ; Paleogenomics 
%B Ancient DNA: Methods and Protocols
%E Shapiro, Beth; Barlow, Axel; Heintzman, Peter D.; Hofreiter, Michael; Paijmans, Johanna L. A.; Soares, André E. R.
%P 25 - 29
%I Springer 
%C New York, NY
%@ 978-1-4939-9176-1
%S Methods in Molecular Biology
%N 1963