%0 Book Section
%A Gansauge, Marie-Theres
%A Meyer, Matthias
%+ Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
%T A method for single-stranded ancient DNA library preparation : 
%G eng
%U https://hdl.handle.net/21.11116/0000-0003-4B3D-6
%R 10.1007/978-1-4939-9176-1_9
%D 2019
%* Review method: peer-reviewed
%X Genomic library preparation from highly degraded DNA is more efficient when library molecules are prepared separately from the complementary strands of DNA fragments. We describe a protocol in which libraries are constructed from single DNA strands in a three-step procedure: single-stranded ligation of the first adapter with T4 DNA ligase in the presence of a splinter oligonucleotide, copying of the DNA strand with a proofreading polymerase, and blunt-end ligation of the second double-stranded adapter with T4 DNA ligase.
%K NGS ; Library preparation ; Ancient DNA ; Single-stranded ligation ; T4 DNA ligase 
%B Ancient DNA: Methods and Protocols
%E Shapiro, Beth; Barlow, Axel; Heintzman, Peter D.; Hofreiter, Michael; Paijmans, Johanna L. A.; Soares, André E. R.
%P 75 - 83
%I Springer 
%C New York, NY
%@ 978-1-4939-9176-1
%S Methods in Molecular Biology
%N 1963