%0 Journal Article
%A Weiß, Clemens L.
%A Gansauge, Marie-Theres
%A Aximu-Petri, Ayinuer
%A Meyer, Matthias
%A Burbano, Hernán A.
%+ Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
%T Mining ancient microbiomes using selective enrichment of damaged DNA molecules : 
%G eng
%U https://hdl.handle.net/21.11116/0000-0006-C11F-E
%R 10.1186/s12864-020-06820-7
%7 2020-06-26
%D 2020
%* Review method: peer-reviewed
%X The identification of bona fide microbial taxa in microbiomes derived from ancient and historical samples is complicated by the unavoidable mixture between DNA from ante- and post-mortem microbial colonizers. One possibility to distinguish between these sources of microbial DNA is querying for the presence of age-associated degradation patterns typical of ancient DNA (aDNA). The presence of uracils, resulting from cytosine deamination, has been detected ubiquitously in aDNA retrieved from diverse sources, and used as an authentication criterion. Here, we employ a library preparation method that separates molecules that carry uracils from those that do not for a set of samples that includes Neandertal remains, herbarium specimens and archaeological plant remains.
%K Ancient DNA ; Metagenomics ; Authentication
%J BMC Genomics
%V 21
%] 432 
%@ 1471-2164