%0 Journal Article
%A Bauer, Judith S.
%A Fillinger, Sven
%A Förstner, Konrad
%A Herbig, Alexander
%A Jones, Adam C.
%A Flinspach, Katrin
%A Sharma, Cynthia
%A Gross, Harald
%A Nieselt, Kay
%A Apel, Alexander K.
%+ Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society
%T dRNA-seq transcriptional profiling of the FK506 biosynthetic gene cluster in Streptomyces tsukubaensis NRRL18488 and general analysis of the transcriptome : 
%G eng
%U https://hdl.handle.net/21.11116/0000-0007-1BC5-D
%R 10.1080/15476286.2017.1341020
%F OTHER: shh2346
%7 2017-07-30
%D 2017
%* Review method: peer-reviewed
%X ABSTRACTFK506 (tacrolimus) is a valuable immunosuppressant produced by several Streptomyces strains. In the genome of the wild type producer Streptomyces tsukubaensis NRRL18488, FK506 biosynthesis is encoded by a gene cluster that spans 83.5 (kb). A whole transcriptome differential shotgun sequencing (dRNA-seq) of S. tsukubaensis was performed to analyze transcription at 2 different time points; before and during active FK506 production. In total, 8,914 transcription start sites were identified in either condition, which enabled precise determination of the 5′-UTR length of the corresponding transcripts as well as the identification of 2 consensus sequence motifs in the promoter regions. The transcription start sites of all gene operons within the FK506 cluster were identified, including 3 examples of leaderless RNA transcripts. These data provide detailed insight into the transcription of the FK506 biosynthetic gene cluster to support future regulatory studies, genetic manipulation, and industrial production.
%K dRNA-seq, FK506, immunosuppressive, Streptomyces, tacrolimus, transcriptome, transcription start sites
%J RNA Biology
%V 14
%N 11
%& 1617
%P 1617 - 1626
%I Landes Bioscience
%C Georgetown, TX
%@ 1547-6286