Relevant papers on ZooMS applications by the Department of Human Evolution can be found at:
We are interested in developing methods for the extraction and analysis of ancient hominin proteomes for phylogenetic and physiological purposes. We have placed particular emphasis on the explicit testing of de novo/error-tolerant bioinformatics algorithms to facilitate the accurate description of novel protein sequences. This has allowed us to provide the first biomolecular sequence data available for a group of extinct South American mammals, to build a collagen type I sequence database facilitating accurate ZooMS identifications, as well as the detection of protein sequence differences between modern humans and Neanderthals.
Working with ancient hominin proteomes requires careful consideration of modern human protein contamination within and outside the laboratory environment.
We actively seek ways to
1) minimize protein contamination during the laboratory work, and
2) to potentially differentiate between contaminating proteins/peptides and endogenous hominin proteins after bioinformatic analysis.
Relevant papers on hominin shotgun proteomics and de novo/error-tolerant ancient protein sequence analysis can be found at: