25.11.2020 - 14:10
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Modern and Archaic Human Cell Biology (Gray Camp)

(Former group hosted by the Department of Evolutionary Genetics)

The group was a member of the Genetics Department from 2017-2019 and has moved to the Institute of Molecular and Clinical Ophthalmology in Basel, Switzerland.

More information on the group can be found here: 
https://www.graycamplab.org/

The group leader's new e-mail contact is: grayson.camp@[>>> Please remove the brackets! <<<]iob.ch

Overview

My group works on methods to recapitulate human, chimpanzee and other great ape development in controlled cell culture environments using three-dimensional (3D) organoids and other stem cell-based systems. We integrate multiple methods including tissue engineering, gene editing, high-throughput confocal microscopy, single-cell genomics, and comparative genomics. We want to understand how human cells are unique from our closest living and extinct relatives, how they vary in human populations around the world, and how they fail in human disease. More detail describing on-going projects can be found below:

1. Great ape organoids

Complex, multi-lineage, 3D organ-like tissues (so called “organoids”) can be generated using pluripotent stem cells from human, chimpanzee and other great apes. We are using single-cell genomics on human, chimp, and other great ape organoids to uncover features that are unique to human developmental programs.

2. Neandertalized cells

We are working with other groups in the department to revert modern human DNA in human stem cells back to the ancestral state to create “Neandertalized” stem cell lines. We will use these cells to understand the physiological impact of genetic change on the modern human lineage.

3. High-throughput cellular phenotyping

We are developing high-throughput strategies to use CRISPR-Cas9 editing in combination with single-cell transcriptomics and confocal imaging to understand how genetic change affects neuron and hepatocyte cell biology.

4. Organ malformation

We are using 3D organoids to understand how rare human genetic diseases perturb gene networks controlling human development, with particular focus on the cortex and liver.

Former Group Staff

  • Jarrett Grayson (Gray) Camp
  • Fátima Sanchis Calleja
  • Leila Sidow
  • Umut Kilik
  • Qianhui Yu
  • Nikolas Kühn

Publications

2020

Dannemann, M., He, Z., Heide, C., Vernot, B., Sidow, L., Kanton, S., Weigert, A., Treutlein, B., Pääbo, S., Kelso, J., & Camp, J. G. (2020). Human stem cell resources are an inroad to Neandertal DNA functions. Stem Cell Reports,15, 214-225.
Open Access    DOI    BibTeX   Endnote   

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He, Z., Gerber, T., Maynard, A., Jain, A., Petri, R., Santel, M., Ly, K., Sidow, L., Sanchís-Calleja, F., Riesenberg, S., Camp, J. G., & Treutlein, B. (2020). Lineage recording reveals dynamics of cerebral organoid regionalization. bioRxiv.
DOI    BibTeX   Endnote   

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Miller, A. J., Yu, Q., Czerwinski, M., Tsai, Y.-H., Conway, R. F., Wu, A., Holloway, E. M., Walker, T., Glass, I. A., Treutlein, B., Camp, J. G., & Spence, J. R. (2020). In vitro and in vivo development of the human airway at single-cell resolution. Developmental Cell,53(1): e6, pp. 117-128.
DOI    BibTeX   Endnote   

2019

Brazovskaja, A., Treutlein, B., & Camp, J. G. (2019). High-throughput single-cell transcriptomics on organoids. Current Opinion in Biotechnology,55, 167-171.
DOI    BibTeX   Endnote   
Kanton, S., Boyle, M. J., He, Z., Santel, M., Weigert, A., Sanchís-Calleja, F., Guijarro, P., Sidow, L., Fleck, J. S., Han, D., Qian, Z., Heide, M., Huttner, W. B., Khaitovich, P., Pääbo, S., Treutlein, B., & Camp, J. G. (2019). Organoid single-cell genomic atlas uncovers human-specific features of brain development. Nature,574(7778), 418-422.
DOI    BibTeX   Endnote   

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Klaus, J., Kanton, S., Kyrousi, C., Ayo-Martin, A. C., Giaimo, R. D., Riesenberg, S., O’Neill, A. C., Camp, J. G., Tocco, C., Santel, M., Rusha, E., Drukker, M., Schroeder, M., Götz, M., Robertson, S. P., Treutlein, B., & Cappello, S. (2019). Altered neuronal migratory trajectories in human cerebral organoids derived from individuals with neuronal heterotopia. Nature Medicine,25, 561-568.
DOI    BibTeX   Endnote   

2018

Camp, J. G., Wollny, D., & Treutlein, B. (2018). Single-cell genomics to guide human stem cell and tissue engineering. Nature Methods,15(9), 661-667.
DOI    BibTeX   Endnote   
Gerber, T., Murawala, P., Knapp, D., Masselink, W., Schuez, M., Hermann, S., Gac-Santel, M., Nowoshilow, S., Kageyama, J., Khattak, S., Currie, J. D., Camp, J. G., Tanaka, E. M., & Treutlein, B. (2018). Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration. Science,362(6413): eaaq0681.
DOI    BibTeX   Endnote   
Kageyama, J., Wollny, D., Treutlein, B., & Camp, J. G. (2018). ShinyCortex: Exploring single-cell transcriptome data from the developing human cortex. Frontiers in Neuroscience,12: 315.
Open Access    DOI    BibTeX   Endnote   

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Karow, M., Camp, J. G., Falk, S., Gerber, T., Pataskar, A., Gac-Santel, M., Kageyama, J., Brazovskaja, A., Garding, A., Fan, W., Riedemann, T., Casamassa, A., Smiyakin, A., Schichor, C., Goetz, M., Tiwari, V. K., Treutlein, B., & Berninger, B. (2018). Direct pericyte-to-neuron reprogramming via unfolding of a neural stem cell-like program. Nature Reviews Neurology,21, 932-940.
DOI    BibTeX   Endnote   
Vaid, S., Camp, J. G., Hersemann, L., Oegema, C. E., Heninger, A.-K., Winkler, S., Brandl, H., Sarov, M., Treutlein, B., Huttner, W. B., & Namba, T. (2018). A novel population of Hopx-dependent basal radial glial cells in the developing mouse neocortex. Development,145(20): dev169276.
DOI    BibTeX   Endnote   

2017

Camp, J. G., & Treutlein, B. (2017). Advances in mini-brain technology. Nature,545(7652), 39-40.
DOI    BibTeX   Endnote   
Camp, J. G., & Treutlein, B. (2017). Human organomics: A fresh approach to understanding human development using single-cell transcriptomics. Development,144(9), 1584-1587.
DOI    BibTeX   Endnote   
Camp, J. G., Sekine, K., Gerber, T., Loeffler-Wirth, H., Binder, H., Gac, M., Kanton, S., Kageyama, J., Damm, G., Seehofer, D., Belicova, L., Bickle, M., Barsacchi, R., Okuda, R., Yoshizawa, E., Kimura, M., Ayabe, H., Taniguchi, H., Takebe, T., & Treutlein, B. (2017). Multilineage communication regulates human liver bud development from pluripotency. Nature,546(7659), 533-538.
DOI    BibTeX   Endnote   
Gerber, T., Willscher, E., Loeffler-Wirth, H., Hopp, L., Schadendorf, D., Schartl, M., Anderegg, U., Camp, G., Treutlein, B., Binder, H., & Kunz, M. (2017). Mapping heterogeneity in patient-derived melanoma cultures by single-cell RNA-seq. Oncotarget,8(1), 846-862.
Open Access    DOI    BibTeX   Endnote   

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2016

Gokce, O., Stanley, G. M., Treutlein, B., Neff, N. F., Camp, J. G., Malenka, R. C., Rothwell, P. E., Fuccillo, M. V., Südhof, T. C., & Quake, S. R. (2016). Cellular taxonomy of the mouse striatum as revealed by single-cell RNA-seq. Cell Reports,16(4), 1126-1137.
Open Access    DOI    BibTeX   Endnote   

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Karow, M., Camp, J. G., Pataskar, A., Schichor, C., Tiwari, V. K., Treutlein, B., & Berninger, B. (2016). Unravelling the molecular synergism of adult human brain pericyte reprogramming into induced GABAergic neurons by Sox2 and Ascl1. Human Gene Therapy,27(11), A169.
DOI    BibTeX   Endnote   
Mora-Bermúdez, F., Badsha, F., Kanton, S., Camp, J. G., Vernot, B., Köhler, K., Voigt, B., Okita, K., Maricic, T., He, Z., Lachmann, R., Pääbo, S., Treutlein, B., & Huttner, W. B. (2016). Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development. eLife, e18683.
Open Access    DOI    BibTeX   Endnote   

Downloads

Treutlein, B., Lee, Q. Y., Camp, J. G., Mall, M., Koh, W., Shariati, S. A. M., Sim, S., Neff, N. F., Skotheim, J. M., Wernig, M., & Quake, S. R. (2016). Dissecting direct reprogramming from fibroblast to neuron using single-cell RNA-seq. Nature,534(7607), 391-395.
DOI    BibTeX   Endnote   

2015

Camp, J. G., Badsha, F., Florio, M., Kanton, S., Gerber, T., Wilsch-Bräuninger, M., Lewitus, E., Sykes, A., Hevers, W., Lancaster, M., Knoblich, J. A., Lachmann, R., Pääbo, S., Huttner, W. B., & Treutlein, B. (2015). Human cerebral organoids recapitulate gene expression programs of fetal neocortex development. Proceedings of the National Academy of Sciences of the United States of America,112(51), 15672-15677.
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