% pubman genre = article
@article{item_3007574,
title = {{Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing}},
author = {Rohland, Nadin and Glocke, Isabelle and Ayinuer-Petri, Aximu and Meyer, Matthias},
language = {eng},
issn = {1750-2799},
doi = {10.1038/s41596-018-0050-5},
publisher = {Nature Publishing Group},
address = {London, UK},
year = {2018},
date = {2018-11},
abstract = {{DNA preserved in ancient bones, teeth and sediments is typically highly fragmented and present only in minute amounts.{\textless}br{\textgreater}Here, we provide a highly versatile silica-based DNA extraction protocol that enables the retrieval of short ($\geq$35 bp) or{\textless}br{\textgreater}even ultrashort ($\geq$25 bp) DNA fragments from such material with minimal carryover of substances that inhibit library{\textless}br{\textgreater}preparation for high-throughput sequencing. DNA extraction can be performed with either silica spin columns, which offer{\textless}br{\textgreater}the most convenient choice for manual DNA extraction, or silica-coated magnetic particles. The latter allow a substantial{\textless}br{\textgreater}cost reduction as well as automation on liquid-handling systems. This protocol update replaces a now-outdated version{\textless}br{\textgreater}that was published 11 years ago, before high-throughput sequencing technologies became widely available. It has been{\textless}br{\textgreater}thoroughly optimized to provide the highest DNA yields from highly degraded samples, as well as fast and easy handling, requiring not more than {\textasciitilde}15 min of hands-on time per sample.{\textless}br{\textgreater}This protocol is an update to: Nat. Protoc. 2, 1756{\textendash}1762 (2007): https://doi.org/10.1038/nprot.2007.247}},
journal = {{Nature Protocols}},
volume = {13},
pages = {2447--2461},
}