% pubman genre = book-item
@incollection{item_3039377,
title = {{A method for single-stranded ancient DNA library preparation}},
author = {Gansauge, Marie-Theres and Meyer, Matthias},
language = {eng},
isbn = {978-1-4939-9176-1},
doi = {10.1007/978-1-4939-9176-1{\textbraceleft}\textunderscore{\textbraceright}9},
publisher = {Springer},
address = {New York, NY},
edition = {2nd ed.},
year = {2019},
date = {2019},
abstract = {{Genomic library preparation from highly degraded DNA is more efficient when library molecules are prepared separately from the complementary strands of DNA fragments. We describe a protocol in which libraries are constructed from single DNA strands in a three-step procedure: single-stranded ligation of the first adapter with T4 DNA ligase in the presence of a splinter oligonucleotide, copying of the DNA strand with a proofreading polymerase, and blunt-end ligation of the second double-stranded adapter with T4 DNA ligase.}},
booktitle = {{Ancient DNA: Methods and Protocols}},
editor = {Shapiro, Beth and Barlow, Axel and Heintzman, Peter D. and Hofreiter, Michael and Paijmans, Johanna L. A. and Soares, Andr{\'e} E. R.},
pages = {75--83},
series = {{Methods in Molecular Biology}},
volume = {1963},
}