% pubman genre = article
@article{item_3221602,
title = {{In vitro and in vivo development of the human airway at single-cell resolution}},
author = {Miller, Alyssa J. and Yu, Qianhui and Czerwinski, Michael and Tsai, Yu-Hwai and Conway, Renee F. and Wu, Angeline and Holloway, Emily M. and Walker, Taylor and Glass, Ian A. and Treutlein, Barbara and Camp, J. Gray and Spence, Jason R.},
language = {eng},
issn = {1534-5807},
doi = {10.1016/j.devcel.2020.01.033},
year = {2020},
date = {2020-04-06},
abstract = {{Summary{\textless}br{\textgreater}Bud tip progenitor cells give rise to all murine lung epithelial lineages and have been described in the developing human lung; however, the mechanisms controlling human bud tip differentiation into specific lineages are unclear. Here, we used homogeneous human bud tip organoid cultures and identified SMAD signaling as a key regulator of the bud tip-to-airway transition. SMAD induction led to the differentiation of airway-like organoids possessing functional basal cells capable of clonal expansion and multilineage differentiation. To benchmark in vitro-derived organoids, we developed a single-cell mRNA sequencing atlas of the human lung from 11.5 to 21 weeks of development, which revealed high degrees of similarity between the in vitro-derived and in vivo airway. Together, this work sheds light on human airway differentiation in vitro and provides a single-cell atlas of the developing human lung.}},
journal = {{Developmental Cell}},
volume = {53},
number = {1},
pages = {117--128},
eid = {e6},
}