% pubman genre = article
@article{item_3255551,
title = {{dRNA-seq transcriptional profiling of the FK506 biosynthetic gene cluster in Streptomyces tsukubaensis NRRL18488 and general analysis of the transcriptome}},
author = {Bauer, Judith S. and Fillinger, Sven and F{\"o}rstner, Konrad and Herbig, Alexander and Jones, Adam C. and Flinspach, Katrin and Sharma, Cynthia and Gross, Harald and Nieselt, Kay and Apel, Alexander K.},
language = {eng},
issn = {1547-6286},
doi = {10.1080/15476286.2017.1341020},
publisher = {Landes Bioscience},
address = {Georgetown, TX},
year = {2017},
date = {2017},
abstract = {{ABSTRACTFK506 (tacrolimus) is a valuable immunosuppressant produced by several Streptomyces strains. In the genome of the wild type producer Streptomyces tsukubaensis NRRL18488, FK506 biosynthesis is encoded by a gene cluster that spans 83.5 (kb). A whole transcriptome differential shotgun sequencing (dRNA-seq) of S. tsukubaensis was performed to analyze transcription at 2 different time points; before and during active FK506 production. In total, 8,914 transcription start sites were identified in either condition, which enabled precise determination of the 5′-UTR length of the corresponding transcripts as well as the identification of 2 consensus sequence motifs in the promoter regions. The transcription start sites of all gene operons within the FK506 cluster were identified, including 3 examples of leaderless RNA transcripts. These data provide detailed insight into the transcription of the FK506 biosynthetic gene cluster to support future regulatory studies, genetic manipulation, and industrial production.}},
journal = {{RNA Biology}},
volume = {14},
number = {11},
pages = {1617--1626},
}