% pubman genre = article
@article{item_3331954,
title = {{Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples}},
author = {Wirobski, G. and Schaebs, F. S. and Range, F. and Marshall-Pescini, S. and Deschner, Tobias},
language = {eng},
issn = {2045-2322},
doi = {10.1038/s41598-021-92356-z},
publisher = {Nature},
year = {2021},
abstract = {{Oxytocin (OT) promotes pro-sociality, bonding, and cooperation in a variety of species. Measuring{\textless}br{\textgreater}oxytocin metabolite (OTM) concentrations in urine or saliva provides intriguing opportunities to study{\textless}br{\textgreater}human and animal behaviour with minimal disturbance. However, a thorough validation of analytical{\textless}br{\textgreater}methods and an assessment of the physiological signifcance of these measures are essential. We{\textless}br{\textgreater}conducted an analytical validation of a commercial Enzyme Immunoassay (EIA; Arbor OT assay kit){\textless}br{\textgreater}to measure OTM concentrations in dog, wolf, and human urine samples. To test the assay{\textquoteright}s ability{\textless}br{\textgreater}to detect changes in OTM concentrations, we administered oxytocin intranasally to 14 dogs. Assay{\textless}br{\textgreater}performance with regard to parallelism was acceptable. Assay accuracy and extraction efciency{\textless}br{\textgreater}for dog and wolf samples were comparable to a previously validated assay (Enzo OT assay kit) but{\textless}br{\textgreater}variation was smaller for human samples. Binding sensitivity and antibody specifcity were better in{\textless}br{\textgreater}the Arbor assay. Average OTM concentrations were more than twice as high as in comparable samples{\textless}br{\textgreater}measured with the Enzo assay, highlighting a lack of comparability of absolute values between{\textless}br{\textgreater}diferent assays. Changes in OTM concentrations after intranasal treatment were detected reliably.{\textless}br{\textgreater}The Arbor assay met requirements of a {\textquotedblleft}ft-for-purpose{\textquotedblright} validation with improvement of several{\textless}br{\textgreater}parameters compared to the Enzo assay.}},
journal = {{Scientific Reports}},
volume = {11},
eid = {12793},
}