% pubman genre = article
@article{item_3338750,
title = {{Using Y-chromosome capture enrichment to resolve haplogroup H2 shows new evidence for a two-path Neolithic expansion to Western Europe}},
author = {Rohrlach, Adam Ben and Papac, Luka and Childebayeva, Ainash and Rivollat, Ma{\"\i}t{\'e} and Villalba-Mouco, Vanessa and Neumann, Gunnar U. and Penske, Sandra and Skourtanioti, Eirini and Loosdrecht, Marieke van de and Akar, Murat and Boyadzhiev, Kamen and Boyadzhiev, Yavor and Deguilloux, Marie-France and Dobe{\v{s}}, Miroslav and Erdal, Yilmaz S. and Ern{\'e}e, Michal and Frangipane, Marcella and Furmanek, Miros{\l}aw and Friederich, Susanne and Ghesqui{\`e}re, Emmanuel and Ha{\l}uszko, Agata and Hansen, Svend and K{\"u}{\ss}ner, Mario and Mannino, Marcello and {\"O}zbal, Rana and Reinhold, Sabine and Rottier, St{\'e}phane and Salazar-Garc{\'\i}a, Domingo Carlos and Diaz, Jorge Soler and Stockhammer, Philipp W. and de Mu{\~n}oz, Togores and Roca, Consuelo and Yener, K. Aslihan and Posth, Cosimo and Krause, Johannes and Herbig, Alexander and Haak, Wolfgang},
language = {eng},
isbn = {2045-2322},
doi = {10.1038/s41598-021-94491-z},
year = {2021},
abstract = {{Uniparentally-inherited markers on mitochondrial DNA (mtDNA) and the non-recombining regions of the Y chromosome (NRY), have been used for the past 30 years to investigate the history of humans from a maternal and paternal perspective. Researchers have preferred mtDNA due to its abundance in the cells, and comparatively high substitution rate. Conversely, the NRY is less susceptible to back mutations and saturation, and is potentially more informative than mtDNA owing to its longer sequence length. However, due to comparatively poor NRY coverage via shotgun sequencing, and the relatively low and biased representation of Y-chromosome variants on capture assays such as the 1240 k, ancient DNA studies often fail to utilize the unique perspective that the NRY can yield. Here we introduce a new DNA enrichment assay, coined YMCA (Y-mappable capture assay), that targets the "mappable" regions of the NRY. We show that compared to low-coverage shotgun sequencing and 1240 k capture, YMCA significantly improves the mean coverage and number of sites covered on the NRY, increasing the number of Y-haplogroup informative SNPs, and allowing for the identification of previously undiscovered variants. To illustrate the power of YMCA, we show that the analysis of ancient Y-chromosome lineages can help to resolve Y-chromosomal haplogroups. As a case study, we focus on H2, a haplogroup associated with a critical event in European human history: the Neolithic transition. By disentangling the evolutionary history of this haplogroup, we further elucidate the two separate paths by which early farmers expanded from Anatolia and the Near East to western Europe.}},
journal = {{Scientific Reports}},
volume = {11},
eid = {15005},
}