% pubman genre = article
@article{item_3521082,
title = {{Efficient high-precision homology-directed repair-dependent genome editing by HDRobust}},
author = {Riesenberg, Stephan and Kanis, Philipp and Macak, Dominik and Wollny, Damian and D{\"u}sterh{\"o}ft, Dorothee and Kowalewski, Johannes and Helmbrecht, Nelly and Maricic, Tomislav and P{\"a}{\"a}bo, Svante},
language = {eng},
issn = {1548-7091; 1548-7105},
doi = {10.1038/s41592-023-01949-1},
year = {2023},
date = {2023},
abstract = {{Homology-directed repair (HDR), a method for repair of DNA {\textless}br{\textgreater}double-stranded breaks can be leveraged for the precise introduction {\textless}br{\textgreater}of mutations supplied by synthetic DNA donors, but remains limited by {\textless}br{\textgreater}low efficiency and off-target effects. In this study, we report HDRobust, {\textless}br{\textgreater}a high-precision method that, via the combined transient inhibition of {\textless}br{\textgreater}nonhomologous end joining and microhomology-mediated end joining, {\textless}br{\textgreater}resulted in the induction of point mutations by HDR in up to 93{\textpercent} (median {\textless}br{\textgreater}60{\textpercent}, s.e.m. 3) of chromosomes in populations of cells. We found that, using {\textless}br{\textgreater}this method, insertions, deletions and rearrangements at the target site, as {\textless}br{\textgreater}well as unintended changes at other genomic sites, were largely abolished. {\textless}br{\textgreater}We validated this approach for 58 different target sites and showed that it {\textless}br{\textgreater}allows efficient correction of pathogenic mutations in cells derived from {\textless}br{\textgreater}patients suffering from anemia, sickle cell disease and thrombophilia.}},
journal = {{Nature Methods}},
volume = {20},
pages = {1388--1399},
}