% pubman genre = article
@article{item_3593829,
title = {{Dietary and homeostatic controls of Zn isotopes in rats: A controlled feeding experiment and modelling approach}},
author = {Bourgon, Nicolas and Tacail, Th{\'e}o and Jaouen, Klervia and Leichliter, Jennifer N and McCormack, Jeremy and Winkler, Daniela E and Clauss, Marcus and T{\"u}tken, Thomas},
language = {eng},
issn = {1756-5901},
doi = {10.1093/mtomcs/mfae026},
year = {2024},
date = {2024-06},
abstract = {{The stable isotope composition of zinc ($\delta$66Zn), which is an essential trace metal for many biological processes in vertebrates, is increasingly used in ecological, archeological, and paleontological studies to assess diet and trophic level discrimination among vertebrates. However, the limited understanding of dietary controls and isotopic fractionation processes on Zn isotope variability in animal tissues and biofluids limits precise dietary reconstructions. The current study systematically investigates the dietary effects on Zn isotope composition in consumers using a combined controlled-feeding experiment and box-modelling approach. For this purpose, 21 rats were fed one of seven distinct animal- and plant-based diets and a total of 148 samples including soft and hard tissue, biofluid, and excreta samples of these individuals were measured for $\delta$66Zn. Relatively constant Zn isotope fractionation is observed across the different dietary groups for each tissue type, implying that diet is the main factor controlling consumer tissue $\delta$66Zn values, independent of diet composition. Furthermore, a systematic $\delta$66Zn diet-enamel fractionation is reported for the first time, enabling diet reconstruction based on $\delta$66Zn values from tooth enamel. In addition, we investigated the dynamics of Zn isotope variability in the body using a box-modelling approach, providing a model of Zn isotope homeostasis and inferring residence times, while also further supporting the hypothesis that $\delta$66Zn values of vertebrate tissues are primarily determined by that of the diet. Altogether this provides a solid foundation for refined (paleo)dietary reconstruction using Zn isotopes of vertebrate tissues.}},
journal = {{Metallomics}},
volume = {16},
number = {6},
eid = {mfae026},
}