%0 Journal Article
%A Mann, Allison E.
%A Fellows Yates, James A.
%A Fagernäs, Zandra
%A Austin, Rita M.
%A Nelson, Elizabeth A.
%A Hofman, Courtney A.
%+ Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society
Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society
Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society
%T Do I have something in my teeth? The trouble with genetic analyses of diet from archaeological dental calculus : 
%G eng
%U https://hdl.handle.net/21.11116/0000-0007-78A8-5
%R 10.1016/j.quaint.2020.11.019
%7 2020-11-19
%D 2023
%8 20.04.2023
%* Review method: peer-reviewed
%X Dental calculus and other preserved microbiome substrates are an attractive target for dietary reconstruction in past populations through a variety of physical, chemical, and molecular means. Recently, studies have attempted to reconstruct diet from archaeological dental calculus using archaeogenetic techniques. While dental calculus may provide a relatively stable environment for DNA preservation, the detection of plants and animals possibly consumed by an individual through DNA analysis is primarily hindered by microbial richness and incomplete reference databases. Moreover, high genomic similarity within eukaryotic groups
%K Diet, Dental calculus, Metagenomics, Ancient DNA
%Z 1. Introduction
2. Materials and methods
3. Results
3.1. Effects of low read counts on damage patterns
3.2. Identification of dietary reads from synthetic datasets
3.3. Identification of dietary reads from real datasets
4. Discussion and conclusions
4.1. Checklist for authors and reviewers
4.1.1. Field/storage contamination
4.1.2. Is the laboratory methodology suitable for aDNA authentication?
4.1.3. Is the database and pipeline suitable?
4.1.4. Is the taxonomic resolution analyzed sufficient for reliable interpretation?
4.1.5. Have unexpected taxa been reported and evaluated?
4.1.6. Have laboratory controls been evaluated alongside samples?
4.1.7. Has evidence of aDNA authenticity been provided?
4.1.8. Is there other evidence supporting that dietary organisms were
consumed?
%J Quaternary International
%V 653-654
%& 33
%P 33 - 46
%I Pergamon
%C Oxford
%@ 1040-6182