%0 Journal Article
%A Essel, Elena
%A Korlević, Petra
%A Meyer, Matthias
%+ Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
%T A method for the temperature-controlled extraction of DNA from ancient bones : 
%G eng
%U https://hdl.handle.net/21.11116/0000-0008-E0F5-6
%R 10.2144/btn-2021-0025
%D 2021
%8 07.06.2021
%* Review method: peer-reviewed
%X We here provide a protocol for the decontamination of ancient bones and teeth that is based on a temperature-controlled, sequential release of DNA. DNA can be extracted from all fractions generated with this method and the fraction with the highest proportion of endogenous DNA identified for further analysis. The protocol proceeds through repeated incubation of the sample powder in phosphate buffer at 37, 60 and 90 °C, followed by the complete lysis of the residual sample powder. As DNA is denatured at high temperature, subsequent DNA extraction and library preparation has to be performed using methods optimized for single-stranded DNA.
%K Ancient DNA, sequential DNA extraction, contamination removal, endogenous DNA, archaeological material
%J Biotechniques
%V 71
%N 1
%& 383
%P 383 - 387
%U https://www.protocols.io/view/a-method-for-the-temperature-controlled-extraction-bje5kjg6