%0 Journal Article
%A Wollny, Damian
%+ Single Cell Genomics, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society
%T Opportunities for single‐cell sequencing in synthetic biology : 
%G eng
%U https://hdl.handle.net/21.11116/0000-0011-BC86-2
%R 10.1002/syst.202000016
%7 2020-06-08
%D 2020
%* Review method: peer-reviewed
%X Single-cell sequencing describes the ability to extract information from a single cell among a heterogeneous mix of cell types by sequencing its DNA or RNA. The development of this technology created tremendous excitement in biology. Akin to gaining higher resolution on a microscope, single-cell sequencing opened our eyes towards previously unknown cellular heterogeneity and the underlying molecular mechanisms. This technology, however, could in principle be applied to any non-living particle capable of carrying nucleotides. This article proposes the application of single-cell sequencing in synthetic biology in order to analyze large numbers of artificially-generated or re-engineered synthetic cells simultaneously. The high-throughput nature of this technology provides the opportunity to test multiple hypotheses in parallel and thus accelerate the pace of discovery. Synthetic biology serves as a particularly exciting example of many overlooked non-canonical applications of single-cell sequencing.
%J ChemSystemsChem
%V 2
%N 6
%] e2000016
%I Wiley-VCH
%C Weinheim